Part:BBa_J147001:Design

RFP inducible device regulated by LuxR and C6-HSL through the Lux promoter

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 740
  Illegal AgeI site found at 852
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 101
  Illegal BsaI.rc site found at 2535
  

Design Notes

LuxR coding sequence and GFP coding sequence are in tandem, both placed after a Ribosome Binding Site sequence.

Source

This composite part has been created using the Biobrick assembly method with parts taken from the Spring 2010 iGEM distribution.